Part:BBa_K931006:Design
An L-Arabinose Controlled Thiamine Binding - Starch Binding Protein Fusion
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 867
Illegal NgoMIV site found at 894
Illegal AgeI site found at 57
Illegal AgeI site found at 277 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The four genes were placed in sequence of each other with the L. Arabinose promoter coming first, then the thiamine-binding protein gene preceded by the starch-binding protein gene coming next, and then the terminator sequences coming last. The linker sequence between each element is ACTAGA.
Source
The four genes come from separate organisms. The L. Arabinose promoter (BBa_K206000) comes from the Registry of Standard Biological Parts and was originally submitted by Group: iGEM09_British_Columbia. Thiamine-binding protein gene comes from the genomic DNA of E. coli and the starch-binding protein gene comes from the genomic DNA of R. oryzae. The two terminator genes (B0010 B0012) come from the Registry of Standard Biological Parts and was originally submitted by Reshma Shetty.